UreC and ZapA virulence genes amplification in clinical specimen of Proteus mirabilis in Bayelsa state, Nigeria
نویسندگان
چکیده
Background and objective: Proteus mirabilis is part of the Enterobacteriaceae family, Gram negative bacterium which typically lives in human gut, means when it causes illness can be a serious bloodstream infection, urinary tract infection or disseminated infection. UreC ZapA virulent genes constitute major pathogenicity this organism as well its resistance to antibiotics. This study was carried out detect presence isolates sourced from Federal Medical Centre Niger Delta University teaching hospital Bayelsa State. Materials methods: A total one hundred forty (140) clinical samples were collected (FMC) Yenagoa Teaching Hospital (NDUTH) Okolobiri, Of 140 collected, 64(45.7%) males while 76(54.3%) females. The Urine, Sputum, High vaginal swab, Urethral Ear swab Wound swab. inoculated different laboratory media incubated at 37°C for 48 hours. Morphological, cultural, biochemical characteristics Polymerase Chain Reaction (PCR) technique noted appropriately. Means corresponding standard deviations calculated continuous data proportions, along with 95% confidence intervals, categorical data. Results: 81 bacterial obtained these samples, 17(20.9%), 22(27.2%), 8(10.0%), 10(12.3%) 24(29.6%) mirabilis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae Staphylococcus aureus respectively. Fifteen (15) 17(20.9%) P. subjected single-plex PCR amplification using specific primers after extraction DNA samples. Out 15 14(93.3%) positive gene 15(100%) gene. Conclusion: present revealed that genes-UreC are highly specimens FMC NDUTH state, thus making them more pathogenic resistant antibiotics curing effect.
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ژورنال
عنوان ژورنال: Journal of microbiology & experimentation
سال: 2021
ISSN: ['2373-437X']
DOI: https://doi.org/10.15406/jmen.2021.09.00317